Pretreatment: Collect no less than 1 million cells and add 200μl cold phosphate buffer solution.

Extract the supernatant for further measurement. Pretreatment: Weight the sample precisely and add the saline with ratio of 1g sample with 9ml saline. Homogenize the mixture in the ice water bath and the centrifuge the homogenate at 4℃, 12000 rpm/min for 5min. Note, for plasma samples, EDTA is not a recommended coagulant. Reagent 4 solution should be prepared right before its usage with the amount needed. Reagent 4 Solution Preparation: Dilute the given reagent 4 with reagent 1 to the 10 times of its original volume. ABTS Solution should be preserved at RT without light and used within 30 min.ģ. ABTS Solution Preparation: Blend Reagent 1, Reagent 2 and Reagent 3 solution with the ratio of 76:5:4 in order to prepare the desired amount of ABTS solution. Reagent 3 Solution Preparation: Dilute the stock solution with double distilled water (DDW) to 1000 times of its original volume.Ģ.

Plate readers with light filters of desired wavelength (405-425nm)ġ. The kit is a ABTS method for the in vitro quantitative measurement of total antioxidant capacity within serum, plasma, tissue homogenate, cells (or cell culture supernates).

Azino 3 kit